Skip to main content
Addgene

AAVS1 KanR foundation lox casette
(Plasmid #132388)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 132388 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    AAVS1-T2A-Puro-Cas9-TREtight
  • Backbone manufacturer
    Addgene
  • Total vector size (bp) 6226
  • Vector type
    Mammalian Expression, Cre/Lox, CRISPR
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    G418 resistance
  • Alt name
    KanR
  • Alt name
    NeoR
  • GenBank ID
    X01702
  • Promoter promoterless

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AsiSI (not destroyed)
  • 3′ cloning site BbvCI (not destroyed)
  • 5′ sequencing primer TCATGGCATCTTCCAGGGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: Plasmid contains a D238Y mutation in NeoR/KanR, which is not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAVS1 KanR foundation lox casette was a gift from Gerold Schmitt-Ulms (Addgene plasmid # 132388 ; http://n2t.net/addgene:132388 ; RRID:Addgene_132388)

  • For your References section:

    Rapid Generation of Human Neuronal Cell Models Enabling Inducible Expression of Proteins-of-interest for Functional Studies. Wang X, Friesen E, Muller I, Lemieux M, Dukart R, Maia IB, Kalia S, Schmitt-Ulms G. Bio Protoc. 2020 May 5;10(9):e3615. doi: 10.21769/BioProtoc.3615. eCollection 2020 May 5. 10.21769/BioProtoc.3615 PubMed 33659578
Related items:
Commonly requested with: