pGD254
(Plasmid
#132375)
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Purposecontains the sequence of mOrange for C-term fusions in the pGGD000 backbone (GreenGate)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 132375 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGGD000
- Backbone size w/o insert (bp) 2682
- Total vector size (bp) 3399
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Vector typeGreenGate Entry plasmid
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemOrange
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Insert Size (bp)717
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/734616v2 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGD254 was a gift from Rüdiger Simon (Addgene plasmid # 132375 ; http://n2t.net/addgene:132375 ; RRID:Addgene_132375) -
For your References section:
Over the rainbow: A practical guide for fluorescent protein selection in plant FRET experiments. Denay G, Schultz P, Hansch S, Weidtkamp-Peters S, Simon R. Plant Direct. 2019 Dec 6;3(12):e00189. doi: 10.1002/pld3.189. eCollection 2019 Dec. 10.1002/pld3.189 PubMed 31844834