ARR3tk-eGFP/SV40-mCherry
(Plasmid #132360)

• Purpose: To measure transcriptional activity of Androgen Receptor
• Depositing Lab: Charles Sawyers
• Publication: Lee et al Elife. 2019 Jan 15;8. pii: 41913. doi: 10.7554/eLife.41913.

Backbone

• Vector backbone: 7TGC
• Backbone manufacturer: Roel Nusse Lab
• Backbone size w/o insert (bp): 8094
• Total vector size (bp): 8626
• Modifications to backbone: 7xtcf binding site of 7TGC replaced with AR binding elements
• Vector type: Lentiviral
• Selectable markers: mCherry

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AR responsive elements
• Species: H. sapiens (human)
• Insert Size (bp): 532
• Entrez Gene: AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GGCCCGAAGGAATAGAAG
• 3′ sequencing primer: AACGGGCCACAACTCCTC

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry