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pZmUBQ:AVRa1-V1
(Plasmid #132357)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 132357 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pZmUBQ:GW-YFP
  • Backbone size w/o insert (bp) 7244
  • Total vector size (bp) 7626
  • Vector type
    Plant Expression
  • Selectable markers
    Basta

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    AVRa1-V1 ∆SP with stop codon
  • Species
    Blumeria graminis f. sp. hordei
  • Insert Size (bp)
    282
  • Promoter pZmUBQ

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

AVRa1-V1 is expressed without signal peptide and without the YFP fusion encoded in the vector

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pZmUBQ:AVRa1-V1 was a gift from Paul Schulze-Lefert (Addgene plasmid # 132357 ; http://n2t.net/addgene:132357 ; RRID:Addgene_132357)

  • For your References section:

    A cell death assay in barley and wheat protoplasts for identification and validation of matching pathogen AVR effector and plant NLR immune receptors. Saur IML, Bauer S, Lu X, Schulze-Lefert P. Plant Methods. 2019 Oct 24;15:118. doi: 10.1186/s13007-019-0502-0. eCollection 2019. 10.1186/s13007-019-0502-0 PubMed 31666804
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