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Addgene

PV521
(Plasmid #132346)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 132346 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PHP66813
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9 R promoter targeting sgRNA1
  • gRNA/shRNA sequence
    unknown
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PV521 was a gift from Joshua Young (Addgene plasmid # 132346 ; http://n2t.net/addgene:132346 ; RRID:Addgene_132346)

  • For your References section:

    The repurposing of type I-E CRISPR-Cascade for gene activation in plants. Young JK, Gasior SL, Jones S, Wang L, Navarro P, Vickroy B, Barrangou R. Commun Biol. 2019 Oct 18;2:383. doi: 10.1038/s42003-019-0637-6. eCollection 2019. 10.1038/s42003-019-0637-6 PubMed 31646186
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