PV384
(Plasmid
#132344)
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PurposeCas9 DsRed targeting sgRNA expression cassette for Zea mays
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 132344 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePHP66813
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCas9 DsRed targeting sgRNA
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gRNA/shRNA sequenceunknown
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PV384 was a gift from Joshua Young (Addgene plasmid # 132344 ; http://n2t.net/addgene:132344 ; RRID:Addgene_132344) -
For your References section:
The repurposing of type I-E CRISPR-Cascade for gene activation in plants. Young JK, Gasior SL, Jones S, Wang L, Navarro P, Vickroy B, Barrangou R. Commun Biol. 2019 Oct 18;2:383. doi: 10.1038/s42003-019-0637-6. eCollection 2019. 10.1038/s42003-019-0637-6 PubMed 31646186