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PV503
(Plasmid #132342)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 132342 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PHP17720
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CasE-AT-CBF1
  • Species
    A. thaliana (mustard weed); CasE (Streptococcus thermophilus); AT-CBF1 (Arabidopsis thaliana)
  • Mutation
    Codon optimize for expression and stability in Zea mays
  • Entrez Gene
    CBF1 (a.k.a. AT4G25490, ATCBF1, C-repeat/DRE binding factor 1, DRE BINDING PROTEIN 1B, DREB1B, T30C3.11, TRANSCRIPTIONAL ACTIVATOR CBF1)
  • Tag / Fusion Protein
    • AT-CBF1 (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There is a D52G mismatch in CasE that does not impact the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PV503 was a gift from Joshua Young (Addgene plasmid # 132342 ; http://n2t.net/addgene:132342 ; RRID:Addgene_132342)

  • For your References section:

    The repurposing of type I-E CRISPR-Cascade for gene activation in plants. Young JK, Gasior SL, Jones S, Wang L, Navarro P, Vickroy B, Barrangou R. Commun Biol. 2019 Oct 18;2:383. doi: 10.1038/s42003-019-0637-6. eCollection 2019. 10.1038/s42003-019-0637-6 PubMed 31646186
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