pAAV-hSyn-ASAP3Kv-WPRE
(Plasmid #132332)

• Purpose: expresses ASAP3Kv(soma-located version of ASAP3, ASAP-family genetically encoded voltage indicator) in mammalian cells, gene delivered by pAAV-hSyn vector
• Depositing Lab: Michael Lin
• Publication: Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23. doi: 10.1016/j.cell.2019.11.004.

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Backbone

• Vector backbone: pAAV-hSyn
• Backbone manufacturer: addgene
• Backbone size w/o insert (bp): 4559
• Total vector size (bp): 6074
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ASAP3Kv
• Alt name: soma-located version of Accelerated Sensor of Action Potentials 3
• Species: Synthetic
• Promoter: hSynapsin

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: gcagcggaggagtc
• 3′ sequencing primer: cattaaagcagcgtatcc

Resource Information

• Supplemental Documents:
  • paav-hsyn-asap3kv-wpre.gb
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry