pDONR221-NPC1L1
(Plasmid #132303)

• Purpose: Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors
• Depositing Labs: RESOLUTE Consortium, Giulio Superti-Furga
• Publication: Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 10.1038/d41573-020-00056-6.

Backbone

• Vector backbone: pDONR221
• Backbone manufacturer: Thermo Fisher Scientific
• Backbone size w/o insert (bp): 2550
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NPC1L1
• Alt name: SLC65A2
• Species: H. sapiens (human)
• Insert Size (bp): 4077
• Entrez Gene: NPC1L1 (a.k.a. LDLCQ7, NPC11L1, SLC65A2)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13-fwd
• 3′ sequencing primer: M13-rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

For more information on the full Resolute plasmid collection, please see www.addgene.org/depositor-collections/re-solute/. Please provide your feedback on this plasmid to the RESOLUTE consortium by filling out their RESOLUTE repository feedback form: https://forms.office.com/Pages/ResponsePage.aspx?id=0e05yklzmkS7rjFGQL4N7z4feCLQvEJAmVcOCM_u885UN1JJRko0Ukg4TVQwNTZLOUxPQVJWT1NHUCQlQCN0PWcu