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Addgene

pMH610
(Plasmid #131862)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131862 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCDFDuet-1
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Streptomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    PhyB(1-651)-RGD-His6-Cys
  • Alt name
    PhyB*
  • Species
    A. thaliana (mustard weed), Synthetic
  • Insert Size (bp)
    2034
  • Promoter T7 promoter-1

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    HO1
  • Species
    Synechocystis PCC 6803
  • Insert Size (bp)
    723
  • Promoter T7 promoter-2

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    PcyA
  • Species
    Synechocystis PCC 6803
  • Insert Size (bp)
    747
  • Promoter T7 promoter-2

Cloning Information for Gene/Insert 3

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TTGTACACGGCCGCATAATC
  • 3′ sequencing primer GGGTTATGCTAGTTATTGC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMH610 was a gift from Wilfried Weber (Addgene plasmid # 131862 ; http://n2t.net/addgene:131862 ; RRID:Addgene_131862)
  • For your References section:

    Production of Phytochromes by High-Cell-Density E. coli Fermentation. Horner M, Gerhardt K, Salavei P, Hoess P, Harrer D, Kaiser J, Tabor JJ, Weber W. ACS Synth Biol. 2019 Sep 26. doi: 10.1021/acssynbio.9b00267. 10.1021/acssynbio.9b00267 PubMed 31526004