NLS-SuperNova
(Plasmid #131858)

• Purpose: Expresses SuperNova fused to nuclear localization signal (NLS)
• Depositing Lab: Geert van den Bogaart
• Publication: Paardekooper et al Int J Mol Sci. 2019 Aug 25;20(17). pii: ijms20174147. doi: 10.3390/ijms20174147.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: CloneTech
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SuperNova
• Species: Synthetic
• Promoter: cmv
• Tags / Fusion Proteins:
  • Myc tag (C terminal on insert)
  • c-myc nuclear localization signal (NLS) (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: CGCAAATGGGCGGTAGGCGTG
• 3′ sequencing primer: GAAATTTGTGATGCTATTGC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Nuclear targeting was achieved by replacing EGFP in pEGFP-C1 with synthetic DNA coding for SuperNova with an N-terminal c-myc nuclear localization signal (NLS) using restriction sites NheI and HindIII. SuperNova sequence is from: Takemoto, K.; Matsuda, T.; Sakai, N.; Fu, D.; Noda, M.; Uchiyama, S.; Kotera, I.; Arai, Y.; Horiuchi, M.; Fukui, K.; et al. SuperNova, a monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation. Sci. Rep. 2013, 3, 2629.