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pmBanana-Hyg-CTCF-NtermNES-N1
(Plasmid #131850)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131850 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAcGFP-Hyg-N1
  • Modifications to backbone
    None
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CTCF
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    927
  • Mutation
    deleted amino acids 796-2181 (the N-terminus is linked to HIV-Rev protein NES )
  • Entrez Gene
    CTCF (a.k.a. CFAP108, FAP108, MRD21)
  • Tag / Fusion Protein
    • mBanana (C terminal on backbone)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmBanana-Hyg-CTCF-NtermNES-N1 was a gift from Mark Minden (Addgene plasmid # 131850 ; http://n2t.net/addgene:131850 ; RRID:Addgene_131850)

  • For your References section:

    NPM1c impedes CTCF functions through cytoplasmic mislocalization in acute myeloid leukemia. Wang AJ, Han Y, Jia N, Chen P, Minden MD. Leukemia. 2020 May;34(5):1278-1290. doi: 10.1038/s41375-019-0681-8. Epub 2019 Dec 12. 10.1038/s41375-019-0681-8 PubMed 31831844
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