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Addgene

pHcRed-NPMMLF1nes-C1
(Plasmid #131849)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131849 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHcRed-C1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NPM1
  • Species
    H. sapiens (human)
  • Mutation
    This is a control deletion contruct of the cytoplasmic mutant NPM1c causing AML. It contains aa.1-175 attached to the MLF1 nuclear export signal (MLF1 aa33-67)
  • Entrez Gene
    NPM1 (a.k.a. B23, NPM)
  • Tag / Fusion Protein
    • HcRed (N terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHcRed-NPMMLF1nes-C1 was a gift from Mark Minden (Addgene plasmid # 131849 ; http://n2t.net/addgene:131849 ; RRID:Addgene_131849)

  • For your References section:

    NPM1c impedes CTCF functions through cytoplasmic mislocalization in acute myeloid leukemia. Wang AJ, Han Y, Jia N, Chen P, Minden MD. Leukemia. 2020 May;34(5):1278-1290. doi: 10.1038/s41375-019-0681-8. Epub 2019 Dec 12. 10.1038/s41375-019-0681-8 PubMed 31831844
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