p5xUAS-Flag-NPM1wt
(Plasmid
#131822)
-
Purpose4-hydroxytamoxifen inducible vector expressing Flag-NPM1wildtype (human) in mammalian cells through transduction
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 131822 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbone5xUAS 4-OHTinducible system
-
Vector typeLentiviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
p5xUAS-Flag-NPM1wt was a gift from Mark Minden (Addgene plasmid # 131822 ; http://n2t.net/addgene:131822 ; RRID:Addgene_131822) -
For your References section:
NPM1c impedes CTCF functions through cytoplasmic mislocalization in acute myeloid leukemia. Wang AJ, Han Y, Jia N, Chen P, Minden MD. Leukemia. 2020 May;34(5):1278-1290. doi: 10.1038/s41375-019-0681-8. Epub 2019 Dec 12. 10.1038/s41375-019-0681-8 PubMed 31831844