pHcRed-NPM1c-C1
(Plasmid
#131821)
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PurposeExpresses HcRed-NPM1c (cytoplasmic mutant, human) in mammalian cells through transient transfection
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 131821 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepHcRed-C1
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNPM1
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SpeciesH. sapiens (human)
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MutationThis is the cytoplasmic mutant causing AML, where a TCTG insertion occurs within exon 12
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Entrez GeneNPM1 (a.k.a. B23, NPM)
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Tag
/ Fusion Protein
- HcRed (N terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: Plasmid contains an E2K mutation in NPM1 of the NPM1c-C1 insert. This mutation is not known to affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHcRed-NPM1c-C1 was a gift from Mark Minden (Addgene plasmid # 131821 ; http://n2t.net/addgene:131821 ; RRID:Addgene_131821) -
For your References section:
NPM1c impedes CTCF functions through cytoplasmic mislocalization in acute myeloid leukemia. Wang AJ, Han Y, Jia N, Chen P, Minden MD. Leukemia. 2020 May;34(5):1278-1290. doi: 10.1038/s41375-019-0681-8. Epub 2019 Dec 12. 10.1038/s41375-019-0681-8 PubMed 31831844
