pHcRed-NPM1wt-C1
(Plasmid
#131818)
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PurposeExpresses HcRed-NPM1wildtype (human) in mammalian cells through transient transfection
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 131818 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHcRed-C1
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHcRed-NPM1wt-C1 was a gift from Mark Minden (Addgene plasmid # 131818 ; http://n2t.net/addgene:131818 ; RRID:Addgene_131818) -
For your References section:
NPM1c impedes CTCF functions through cytoplasmic mislocalization in acute myeloid leukemia. Wang AJ, Han Y, Jia N, Chen P, Minden MD. Leukemia. 2020 May;34(5):1278-1290. doi: 10.1038/s41375-019-0681-8. Epub 2019 Dec 12. 10.1038/s41375-019-0681-8 PubMed 31831844