pBW005
(Plasmid
#131806)
-
PurposeVector with aTc inducible M. laminosus Fd1 with a C-terminal fused mRFP1
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 131806 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneColE1
- Total vector size (bp) 3917
-
Vector typeSynthetic Biology
Growth in Bacteria
-
Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameMastigocladus laminosus ferredoxin
-
SpeciesSynthetic
-
Insert Size (bp)300
-
Tag
/ Fusion Protein
- mRFP1 (C terminal on insert)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For detailed annotations see the GenBank file under Resource Information/Supplemental Documents. Please visit https://www.biorxiv.org/content/10.1101/645978v1 for BioRxiv preprint
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pBW005 was a gift from Jonathan Silberg (Addgene plasmid # 131806 ; http://n2t.net/addgene:131806 ; RRID:Addgene_131806) -
For your References section:
Combinatorial design of chemical-dependent protein switches for controlling intracellular electron transfer. Wu B, Atkinson JT, Kahanda D, Bennett GN, Silberg JJ. BioRxiv May 23, 2019 10.1101/645978v1