pAcGFP-Hyg-CTCFdZnF-N1
(Plasmid #131803)

• Purpose: Expresses the N and C terminus of human CTCF-GFP with the zinc finger domain removed in mammalian cells
• Depositing Lab: Mark Minden
• Publication: Wang et al Leukemia. 2020 May;34(5):1278-1290. doi: 10.1038/s41375-019-0681-8. Epub 2019 Dec 12.

Backbone

• Vector backbone: pAcGFP-Hyg-N1
• Backbone size w/o insert (bp): 1299
• Total vector size (bp): 7531
• Modifications to backbone: None
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CTCF
• Species: H. sapiens (human)
• Insert Size (bp): 450
• Mutation: deleted amino acids 1-1731
• Entrez Gene: CTCF (a.k.a. CFAP108, FAP108, MRD21)
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Made with the blood and sweat of Atom Wang