pAcGFP-Hyg-CTCF-ZnF-N1
(Plasmid
#131802)
-
PurposeExpresses the [Zinc finger domain of human CTCF (a.a. 265-603)]-GFP in mammalian cells
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 131802 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepAcGFP-Hyg-N1
- Backbone size w/o insert (bp) 1019
- Total vector size (bp) 7251
-
Modifications to backboneNone
-
Vector typeMammalian Expression
-
Selectable markersHygromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCTCF
-
SpeciesH. sapiens (human)
-
Insert Size (bp)450
-
Mutationdeleted amino acids 1-1731
-
Entrez GeneCTCF (a.k.a. CFAP108, FAP108, MRD21)
-
Tag
/ Fusion Protein
- GFP (C terminal on backbone)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Made with the blood and sweat of Atom Wang
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pAcGFP-Hyg-CTCF-ZnF-N1 was a gift from Mark Minden (Addgene plasmid # 131802 ; http://n2t.net/addgene:131802 ; RRID:Addgene_131802) -
For your References section:
NPM1c impedes CTCF functions through cytoplasmic mislocalization in acute myeloid leukemia. Wang AJ, Han Y, Jia N, Chen P, Minden MD. Leukemia. 2020 May;34(5):1278-1290. doi: 10.1038/s41375-019-0681-8. Epub 2019 Dec 12. 10.1038/s41375-019-0681-8 PubMed 31831844