pAcGFP-Hyg-CTCF-Nterm-N1
(Plasmid #131800)

• Purpose: Expresses the [N-terminus of human CTCF (a.a. 2-265)]-GFP in mammalian cells
• Depositing Lab: Mark Minden
• Publication: Wang et al Leukemia. 2020 May;34(5):1278-1290. doi: 10.1038/s41375-019-0681-8. Epub 2019 Dec 12.

Backbone

• Vector backbone: pAcGFP-Hyg-N1
• Backbone size w/o insert (bp): 5791
• Total vector size (bp): 6682
• Modifications to backbone: None
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CTCF
• Species: H. sapiens (human)
• Insert Size (bp): 927
• Mutation: deleted amino acids 796-2181 (the N-terminus is linked to CTCF NLS using a linker peptide)
• Entrez Gene: CTCF (a.k.a. CFAP108, FAP108, MRD21)
• Tags / Fusion Proteins:
  • GFP (C terminal on insert)
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: 50bp upstream of HINDIII site

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Made with the blood and sweat of Atom Wang