pAcGFP-Hyg-CTCF-N1
(Plasmid #131799)

• Purpose: Expresses human CTCF-GFP in mammalian cells through transient transfection
• Depositing Lab: Mark Minden
• Publication: Wang et al Leukemia. 2020 May;34(5):1278-1290. doi: 10.1038/s41375-019-0681-8. Epub 2019 Dec 12.

Backbone

• Vector backbone: pAcGFP-Hyg-N1
• Backbone size w/o insert (bp): 5791
• Total vector size (bp): 7936
• Modifications to backbone: None
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CTCF
• Species: H. sapiens (human)
• Insert Size (bp): 2181
• Entrez Gene: CTCF (a.k.a. CFAP108, FAP108, MRD21)
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: GGAG GTCTATATAA GCAGAGCTG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr Elena Pugacheva

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: Plasmid contains an IS4-like element between the AmpR promoter and AmpR resistance gene. This discrepancy is not known to affect plasmid function as plasmid was able to grow in Ampicillin.