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Addgene

pFA0057
(Plasmid #131784)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131784 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pML107 (addgene #67639)
  • Backbone size w/o insert (bp) 12364
  • Total vector size (bp) 12384
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    20mer GFP cassette guide (gGFP) and 5' sgRNA
  • Alt name
    gRNA sequence - CCCGGGTTAATTAACAGTAA
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    20

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BclI (destroyed during cloning)
  • 3′ cloning site SwaI (destroyed during cloning)
  • 5′ sequencing primer M13 Reverse or T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFA0057 was a gift from Frank Albert (Addgene plasmid # 131784 ; http://n2t.net/addgene:131784 ; RRID:Addgene_131784)

  • For your References section:

    DNA variants affecting the expression of numerous genes in trans have diverse mechanisms of action and evolutionary histories. Lutz S, Brion C, Kliebhan M, Albert FW. PLoS Genet. 2019 Nov 18;15(11):e1008375. doi: 10.1371/journal.pgen.1008375. eCollection 2019 Nov. 10.1371/journal.pgen.1008375 PubMed 31738765
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