EZ-L498
(Plasmid
#131771)
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PurposePPGK1_FUSN_Citrine _PixE_TCYC1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 131771 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneYeast Expression
- Total vector size (bp) 9606
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Vector typeYeast Expression ; Integration into HIS3 locus
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePPGK1_FUSN_Citrine _PixE_TCYC1
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SpeciesSynthetic
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Insert Size (bp)2523
- Promoter PGK
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EZ-L498 was a gift from Jared Toettcher (Addgene plasmid # 131771 ; http://n2t.net/addgene:131771 ; RRID:Addgene_131771) -
For your References section:
Light-based control of metabolic flux through assembly of synthetic organelles. Zhao EM, Suek N, Wilson MZ, Dine E, Pannucci NL, Gitai Z, Avalos JL, Toettcher JE. Nat Chem Biol. 2019 Jun;15(6):589-597. doi: 10.1038/s41589-019-0284-8. Epub 2019 May 13. 10.1038/s41589-019-0284-8 PubMed 31086330