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Addgene

INPP5A / type I IP3 5-phosphatase from dog thyroid
(Plasmid #131767)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131767 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript SK+
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2958
  • Total vector size (bp) 5607
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    INPP5A
  • Alt name
    Type I IP3 5-phosphatase
  • Species
    Dog
  • Insert Size (bp)
    2659
  • GenBank ID
    X75094 NM_001003257.1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T3 Primer
  • 3′ sequencing primer T7 primer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    INPP5A / type I IP3 5-phosphatase from dog thyroid was a gift from Christophe Erneux (Addgene plasmid # 131767 ; http://n2t.net/addgene:131767 ; RRID:Addgene_131767)

  • For your References section:

    Cloning and expression in Escherichia coli of a dog thyroid cDNA encoding a novel inositol 1,4,5-trisphosphate 5-phosphatase. Verjans B, De Smedt F, Lecocq R, Vanweyenberg V, Moreau C, Erneux C. Biochem J. 1994 May 15;300 ( Pt 1):85-90. doi: 10.1042/bj3000085. 10.1042/bj3000085 PubMed 8198557
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