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Addgene

pFH25
(Plasmid #131764)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131764 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pICH41308
  • Vector type
    Plant Expression, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpCas9, wheat codon optimized, version 3
  • Species
    Synthetic

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFH25 was a gift from Vladimir Nekrasov (Addgene plasmid # 131764 ; http://n2t.net/addgene:131764 ; RRID:Addgene_131764)

  • For your References section:

    A modular cloning toolkit for genome editing in plants. Hahn F, Korolev A, Sanjurjo Loures L, Nekrasov V. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. 10.1186/s12870-020-02388-2 PubMed 32326901
Included in kit:
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