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Addgene

pLenti_TMv2_Zeo
(Plasmid #131762)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131762 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti (Addgene #61427)
  • Backbone size (bp) 8000
  • Vector type
    Mammalian Expression, Lentiviral
  • Promoter EF1a
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/762708v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti_TMv2_Zeo was a gift from Paul Blainey (Addgene plasmid # 131762 ; http://n2t.net/addgene:131762 ; RRID:Addgene_131762)

  • For your References section:

    CloneSifter: enrichment of rare clones from heterogeneous cell populations. Feldman D, Tsai F, Garrity AJ, O'Rourke R, Brenan L, Ho P, Gonzalez E, Konermann S, Johannessen CM, Beroukhim R, Bandopadhayay P, Blainey PC. BMC Biol. 2020 Nov 24;18(1):177. doi: 10.1186/s12915-020-00911-3. 10.1186/s12915-020-00911-3 PubMed 33234154
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