pLenti_TMv2
(Plasmid
#131761)
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Purpose(Empty Backbone) frameshift reporter for barcode retrieval
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 131761 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLenti (Addgene #61427)
- Backbone size (bp) 8000
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/762708v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti_TMv2 was a gift from Paul Blainey (Addgene plasmid # 131761 ; http://n2t.net/addgene:131761 ; RRID:Addgene_131761) -
For your References section:
CloneSifter: enrichment of rare clones from heterogeneous cell populations. Feldman D, Tsai F, Garrity AJ, O'Rourke R, Brenan L, Ho P, Gonzalez E, Konermann S, Johannessen CM, Beroukhim R, Bandopadhayay P, Blainey PC. BMC Biol. 2020 Nov 24;18(1):177. doi: 10.1186/s12915-020-00911-3. 10.1186/s12915-020-00911-3 PubMed 33234154