IX001: pBS/Fiber
(Plasmid
#131754)
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PurposePlasmid containing Ad5 Fiber gene flanked by homology arms for insertion into the recombinant Ad5 vector IQ013 to generate tropism modified vectors.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 131754 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBlueScript2SK(-)
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 3000
- Total vector size (bp) 5055
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAd5 wild-type Fiber
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Alt nameL5
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SpeciesAd5
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Insert Size (bp)2242
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GenBank IDM18369.1
- Promoter N/A
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer M13F (-20)
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid is a shuttle vector for the Ad5 Fiber that is engineered to be recombined into the pMVP recombinant Ad5 backbone (IQ013) to generate fiber-modified Ad variants.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
IX001: pBS/Fiber was a gift from Christopher Newgard (Addgene plasmid # 131754 ; http://n2t.net/addgene:131754 ; RRID:Addgene_131754) -
For your References section:
Creation of versatile cloning platforms for transgene expression and dCas9-based epigenome editing. Haldeman JM, Conway AE, Arlotto ME, Slentz DH, Muoio DM, Becker TC, Newgard CB. Nucleic Acids Res. 2018 Dec 27. pii: 5264291. doi: 10.1093/nar/gky1286. 10.1093/nar/gky1286 PubMed 30590691