JI401: Ad5 E1A/Hexon qRT-PCR standard
(Plasmid #131753)

• Purpose: Plasmid standard containing regions of the Ad5 E1A and Ad5 Hexon genes to create a qRT-PCR standard curve for detection of replication competent adenovirus (RCA).
• Depositing Lab: Christopher Newgard
• Publication: Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 5264291. doi: 10.1093/nar/gky1286.

Backbone

• Vector backbone: pBlueScript2SK(-)
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 3325
• Vector type: Standard for qRT-PCR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Ad5 E1A gene fragment
• Species: Ad5
• Insert Size (bp): 200
• Promoter: N/A

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: M13F

Gene/Insert 2

• Gene/Insert name: Ad5 Hexon gene fragment
• Species: Ad5
• Insert Size (bp): 200
• Promoter: N/A

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: M13R

Resource Information

• Supplemental Documents:
  • JI401_E1A_Hexon_qRTPCR_STD.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is a qRT-PCR standard that enables quantification of RCA in preparations of recombinant Ad5. Please see attached protocol for more details.