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Addgene

pAWp63-clone3-12 (OptiHF-SpCas9)
(Plasmid #131737)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131737 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFUGW
  • Total vector size (bp) 13045
  • Vector type
    Lentiviral
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    OptiHF-SpCas9
  • Species
    Synthetic
  • Insert Size (bp)
    4716
  • Mutation
    Q695A+K848A+E923M+T924V+Q926A
  • Promoter EFS
  • Tag / Fusion Protein
    • Flag

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site - (unknown if destroyed)
  • 3′ cloning site - (unknown if destroyed)
  • 5′ sequencing primer GGCTCCGATCAGGTTCTTCTTGATGC
  • 3′ sequencing primer CATAGCGTAAAAGGAGCAACA
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The gene sequence was originally cloned by Dr. Feng Zhang’s group at MIT, which have been deposited the plasmid to Addgene (#49535 and #71814), and were used as the backbone to generate mutations and derive the plasmids deposited by us.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAWp63-clone3-12 (OptiHF-SpCas9) was a gift from Alan Siu Lun Wong (Addgene plasmid # 131737 ; http://n2t.net/addgene:131737 ; RRID:Addgene_131737)

  • For your References section:

    Combinatorial mutagenesis en masse optimizes the genome editing activities of SpCas9. Choi GCG, Zhou P, Yuen CTL, Chan BKC, Xu F, Bao S, Chu HY, Thean D, Tan K, Wong KH, Zheng Z, Wong ASL. Nat Methods. 2019 Aug;16(8):722-730. doi: 10.1038/s41592-019-0473-0. Epub 2019 Jul 15. 10.1038/s41592-019-0473-0 PubMed 31308554
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