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pTransgenesis p3 sv40 minimal promoter:: Katushka RFP
(Plasmid #131731)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131731 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    katushka-RFP-p3-13
  • Backbone manufacturer
    Xenopus Resource
  • Backbone size w/o insert (bp) 3715
  • Total vector size (bp) 3953
  • Modifications to backbone
    Inserted SV40MinPromoter
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SV40MinPromoter
  • Insert Size (bp)
    238

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer M13 fwd
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTransgenesis p3 sv40 minimal promoter:: Katushka RFP was a gift from Rene Maehr (Addgene plasmid # 131731 ; http://n2t.net/addgene:131731 ; RRID:Addgene_131731)

  • For your References section:

    Combinatorial action of NF-Y and TALE at embryonic enhancers defines distinct gene expression programs during zygotic genome activation in zebrafish. Stanney W 3rd, Ladam F, Donaldson IJ, Parsons TJ, Maehr R, Bobola N, Sagerstrom CG. Dev Biol. 2020 Mar 15;459(2):161-180. doi: 10.1016/j.ydbio.2019.12.003. Epub 2019 Dec 17. 10.1016/j.ydbio.2019.12.003 PubMed 31862379
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