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pLenti-puro /BRAF-V600E
(Plasmid #131724)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131724 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti-puro (Plasmid #39481)
  • Backbone manufacturer
    Ie-Ming Shih (Addgene plasmid # 39481)
  • Backbone size w/o insert (bp) 7066
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BRAF
  • Alt name
    B-RAF
  • Alt name
    ENSG00000157764
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2301
  • Mutation
    V600E
  • Entrez Gene
    BRAF (a.k.a. B-RAF1, B-raf, BRAF-1, BRAF1, NS7, RAFB1)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xFLAG (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-puro /BRAF-V600E was a gift from Jacques De Grève (Addgene plasmid # 131724 ; http://n2t.net/addgene:131724 ; RRID:Addgene_131724)

  • For your References section:

    CRAF mutations in lung cancer can be oncogenic and predict sensitivity to combined type II RAF and MEK inhibition. Noeparast A, Giron P, Noor A, Bahadur Shahi R, De Brakeleer S, Eggermont C, Vandenplas H, Boeckx B, Lambrechts D, De Greve J, Teugels E. Oncogene. 2019 Aug;38(31):5933-5941. doi: 10.1038/s41388-019-0866-7. Epub 2019 Jul 8. 10.1038/s41388-019-0866-7 PubMed 31285551
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