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pIRES2-smGFP-Kv2.1
(Plasmid #131709)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131709 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRES2-DsRed-Express
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5300
  • Total vector size (bp) 7982
  • Modifications to backbone
    DsRed-Express was replaced with smGFP by Gibson Assembly
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KCNB1
  • Alt name
    potassium voltage-gated channel subfamily B member 1
  • Alt name
    Kv2.1
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_004975
  • Entrez Gene
    KCNB1 (a.k.a. DRK1, Kv2.1)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (destroyed during cloning)
  • 3′ cloning site NheI (destroyed during cloning)
  • 5′ sequencing primer CMV_fwd_primer
  • 3′ sequencing primer dsRed1_N_primer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIRES2-smGFP-Kv2.1 was a gift from Jennifer Kearney (Addgene plasmid # 131709 ; http://n2t.net/addgene:131709 ; RRID:Addgene_131709)

  • For your References section:

    Spectrum of KV 2.1 Dysfunction in KCNB1-Associated Neurodevelopmental Disorders. Kang SK, Vanoye CG, Misra SN, Echevarria DM, Calhoun JD, O'Connor JB, Fabre KL, McKnight D, Demmer L, Goldenberg P, Grote LE, Thiffault I, Saunders C, Strauss KA, Torkamani A, van der Smagt J, van Gassen K, Carson RP, Diaz J, Leon E, Jacher JE, Hannibal MC, Litwin J, Friedman NR, Schreiber A, Lynch B, Poduri A, Marsh ED, Goldberg EM, Millichap JJ, George AL Jr, Kearney JA. Ann Neurol. 2019 Dec;86(6):899-912. doi: 10.1002/ana.25607. Epub 2019 Oct 24. 10.1002/ana.25607 PubMed 31600826

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