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Addgene

pLKO-M7-puro
(Plasmid #131697)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131697 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLKO
  • Total vector size (bp) 7034
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    M7-puro
  • Species
    MHV68
  • Insert Size (bp)
    249
  • Entrez Gene
    GAMMAHV.M7 (a.k.a. MuHV4gp49)
  • Promoter M7

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GGAGGCTTGGTAGGTTTAAGAA
  • 3′ sequencing primer GGCTTGTACTCGGTCATGGT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/585984v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLKO-M7-puro was a gift from Britt Glaunsinger (Addgene plasmid # 131697 ; http://n2t.net/addgene:131697 ; RRID:Addgene_131697)

  • For your References section:

    Xrn1 activity broadly represses RNA polymerase II occupancy at mammalian but not viral promoters during herpesvirus infection. Hartenian EN, Glaunsinger BA. bioRxiv 585984 10.1101/585984
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