pLVX.TRE3G.AdProt
(Plasmid #131687)

• Purpose: A lentiviral vector for tTA-regulated expression of the adenovirus protease using the tetracycline transactivator transcription factor.
• Depositing Lab: Matthew D. Shoulders
• Publication: Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093-18103. doi: 10.1021/jacs.8b10937. Epub 2018 Dec 14.

Backbone

• Vector backbone: pLVX.TRE3G
• Backbone manufacturer: Takara Bio
• Backbone size w/o insert (bp): 7781
• Total vector size (bp): 8632
• Vector type: Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Adenovirus Protease
• Alt name: adenain
• Species: Adenovirus type 5
• Insert Size (bp): 851
• Promoter: TRE3GV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: ATCGATGAGGCCCTTTC
• 3′ sequencing primer: ggtaagcttgggctgcagg

Resource Information

• Supplemental Documents:
  • pLVX.TRE3G.AdProt.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Tet Systems Limited Use Label License
• Industry Terms:
  • Not Available to Industry