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Addgene

EP-Pol vector
(Plasmid #131686)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131686 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRRL-cPPT-X-PRE-SIN
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Adenovirus DNA polymerase
  • Alt name
    E2B DNA polymerase
  • Species
    Adenovirus type 5
  • Mutation
    F421Y, D827A
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EP-Pol vector was a gift from Matthew D. Shoulders (Addgene plasmid # 131686 ; http://n2t.net/addgene:131686 ; RRID:Addgene_131686)

  • For your References section:

    An Adaptable Platform for Directed Evolution in Human Cells. Berman CM, Papa LJ 3rd, Hendel SJ, Moore CL, Suen PH, Weickhardt AF, Doan ND, Kumar CM, Uil TG, Butty VL, Hoeben RC, Shoulders MD. J Am Chem Soc. 2018 Dec 26;140(51):18093-18103. doi: 10.1021/jacs.8b10937. Epub 2018 Dec 14. 10.1021/jacs.8b10937 PubMed 30427676
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