Wild-type AdPol vector
(Plasmid
#131685)
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PurposeA lentiviral vector that constitutively expresses the wild-type adenovirus DNA polymerase
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 131685 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRRL-cPPT-X-PRE-SIN
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAdenovirus DNA polymerase
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Alt nameE2B DNA polymerase
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SpeciesAdenovirus type 5
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byDr. Robert C. Hoeben, Leiden University Medical Center, Leiden, The Netherlands
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Uil, T. G.; Vellinga, J.; de Vrij, J.; van den Hengel, S. K.; Rabelink, M. J. W. E.; Cramer, S. J.; Eekels, J. J. M.; Ariyurek, Y.; van Galen, M.; Hoeben, R. C. Directed adenovirus evolution using engineered mutator viral polymerases. Nucleic Acids Res. 2011, 39, e30.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Wild-type AdPol vector was a gift from Matthew D. Shoulders (Addgene plasmid # 131685 ; http://n2t.net/addgene:131685 ; RRID:Addgene_131685) -
For your References section:
An Adaptable Platform for Directed Evolution in Human Cells. Berman CM, Papa LJ 3rd, Hendel SJ, Moore CL, Suen PH, Weickhardt AF, Doan ND, Kumar CM, Uil TG, Butty VL, Hoeben RC, Shoulders MD. J Am Chem Soc. 2018 Dec 26;140(51):18093-18103. doi: 10.1021/jacs.8b10937. Epub 2018 Dec 14. 10.1021/jacs.8b10937 PubMed 30427676