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SPIB_pet28a
(Plasmid #131655)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131655 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pet28a
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 5369
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SPIB
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    788
  • Entrez Gene
    SPIB (a.k.a. SPI-B)
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nco1 (unknown if destroyed)
  • 3′ cloning site EcoR1 (unknown if destroyed)
  • 5′ sequencing primer T7 Fwd
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Insert templated from GST-SPIB plasmid from Celeste Simon's lab

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

insert equivalent to CDS in NM_003121 plus 6xHis added with cloning primer

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SPIB_pet28a was a gift from Peter Hollenhorst (Addgene plasmid # 131655 ; http://n2t.net/addgene:131655 ; RRID:Addgene_131655)

  • For your References section:

    Comparison of MAPK specificity across the ETS transcription factor family identifies a high-affinity ERK interaction required for ERG function in prostate cells. Selvaraj N, Kedage V, Hollenhorst PC. Cell Commun Signal. 2015 Feb 19;13:12. doi: 10.1186/s12964-015-0089-7. 10.1186/s12964-015-0089-7 PubMed 25885538
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