pCMV-APOBEC1-YTHmut
(Plasmid #131637)

• Purpose: Expresses APOBEC1 fused to a mutant YTH domain with diminished m6A binding
• Depositing Lab: Kate Meyer
• Publication: Meyer et al Nat Methods. 2019 Sep 23. pii: 10.1038/s41592-019-0570-0. doi: 10.1038/s41592-019-0570-0.

Backbone

• Vector backbone: pCMV-BE1
• Backbone size w/o insert (bp): 3373
• Total vector size (bp): 4651
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: APOBEC1-YTHmut-HA
• Species: H. sapiens (human), R. norvegicus (rat)
• Insert Size (bp): 1278
• Mutation: YTH domain lacks AA 385-409 comprising the m6A-binding region
• Entrez Gene: YTHDF2 (a.k.a. CAHL, DF2, HGRG8, NY-REN-2)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XmaI (not destroyed)
• 3′ cloning site: PmeI (not destroyed)
• 5′ sequencing primer: T7 Fwd
• 3′ sequencing primer: BGH Rev

Resource Information

• Supplemental Documents:
  • pCMV_APOBEC1_YTHmut_HA.gb
• A portion of this plasmid was derived from a plasmid made by: Addgene 73019; pCMV-BE1
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry