Opie2-NeoR[traF]+Opie2-PuroR[dsxM]
(Plasmid #131618)

• Purpose: Expresses a functional NeoR only in females, a functional PuroR only in males, and dsRed in both genders.
• Depositing Lab: Omar Akbari
• Publication: Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi: 10.1038/s41467-020-16020-2.

Backbone

• Vector backbone: PiggyBac
• Backbone size w/o insert (bp): 5200
• Total vector size (bp): 15465
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: PuroR[dsxM]
• Alt name: PuroR with Dsx male-specific intron encoded into PuroR CSD.
• Species: D. melanogaster (fly)
• Insert Size (bp): 5680
• Promoter: Opie2

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CCAAGTTTTAATCGCATAACTTCTGTTAATCCCC
• 3′ sequencing primer: ggtgtcaggcaccgggcttgcgggtc

Gene/Insert 2

• Gene/Insert name: NeoR[traF]
• Alt name: NeoR with Tra female-specific intron encoded into NeoR CDS.
• Species: D. melanogaster (fly)
• Insert Size (bp): 1043
• Promoter: Opie2

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: atgGTACCCACTATATAGTATAACCGATCTG
• 3′ sequencing primer: gatctTTAGAAGAACTCATCCAGCAAGCGGTAG

Resource Information

• Supplemental Documents:
  • Opie2-NeoR[traF]+Opie2-PuroR[dsxM]

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry