Opie2-PuroR
(Plasmid
#131613)
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PurposeExpresses PuroR and dsRed.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 131613 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePiggyBac
- Backbone size w/o insert (bp) 5200
- Total vector size (bp) 8632
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert namePuroR
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SpeciesSynthetic
- Promoter Opie2
Cloning Information for Gene/Insert 1
- Cloning method Gibson Cloning
- 5′ sequencing primer atgactcatacttgattgtgttttacgcggtttaaac
- 3′ sequencing primer TCGAGGTCGAagatctctcaggcaccg (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert namedsRed
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SpeciesSynthetic
- Promoter hr5Ie1
Cloning Information for Gene/Insert 2
- Cloning method Gibson Cloning
- 5′ sequencing primer actggcggcgacaagatcgtgaacaaccaagtg
- 3′ sequencing primer gcggccgctacaggaacaggtggtg (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Opie2-PuroR was a gift from Omar Akbari (Addgene plasmid # 131613 ; http://n2t.net/addgene:131613 ; RRID:Addgene_131613) -
For your References section:
A drug-inducible sex-separation technique for insects. Kandul NP, Liu J, Hsu AD, Hay BA, Akbari OS. Nat Commun. 2020 Apr 30;11(1):2106. doi: 10.1038/s41467-020-16020-2. 10.1038/s41467-020-16020-2 PubMed 32355156
Map uploaded by the depositor.
