Skip to main content
Addgene

ZapLck biosensor
(Plasmid #131584)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131584 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pSIN-EF2-Nanog-Pur
  • Backbone manufacturer
    Addgene (#16578)
  • Backbone size w/o insert (bp) 7575
  • Total vector size (bp) 9378
  • Modifications to backbone
    The insert is between SpeI and EcoRI.
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ZapLck biosensor
  • Alt name
    Lck biosensor
  • Species
    Synthetic
  • Insert Size (bp)
    1803

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ZapLck biosensor was a gift from Yingxiao Wang (Addgene plasmid # 131584 ; http://n2t.net/addgene:131584 ; RRID:Addgene_131584)

  • For your References section:

    Biophysical basis underlying dynamic Lck activation visualized by ZapLck FRET biosensor. Wan R, Wu J, Ouyang M, Lei L, Wei J, Peng Q, Harrison R, Wu Y, Cheng B, Li K, Zhu C, Tang L, Wang Y, Lu S. Sci Adv. 2019 Jun 19;5(6):eaau2001. doi: 10.1126/sciadv.aau2001. eCollection 2019 Jun. 10.1126/sciadv.aau2001 PubMed 31223643
Related items:
Commonly requested with: