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Addgene

AdMKP-3 S197A
(Plasmid #131522)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131522 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAd/CMV/V5-DEST
  • Backbone manufacturer
    Invitrogen (Life Technologies)
  • Backbone size w/o insert (bp) 36686
  • Total vector size (bp) 37832
  • Modifications to backbone
    No
  • Vector type
    Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Mitogen-activated protein kinase phosphatase 3
  • Alt name
    Mkp-3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1146
  • Mutation
    changed Serine 197 to Alanine
  • GenBank ID
    NM_026268.3
  • Entrez Gene
    Dusp6 (a.k.a. 1300019I03Rik, MKP-3, MKP3, PYST1)
  • Promoter CMV
  • Tag / Fusion Protein
    • No

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AdMKP-3 S197A was a gift from Haiyan Xu (Addgene plasmid # 131522 ; http://n2t.net/addgene:131522 ; RRID:Addgene_131522)

  • For your References section:

    MEK/ERK pathway mediates insulin-promoted degradation of MKP-3 protein in liver cells. Feng B, Jiao P, Yang Z, Xu H. Mol Cell Endocrinol. 2012 Sep 25;361(1-2):116-23. doi: 10.1016/j.mce.2012.03.025. Epub 2012 Apr 12. 10.1016/j.mce.2012.03.025 PubMed 22521266
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