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Addgene

pET30-RT1306
(Plasmid #131521)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131521 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET30
  • Backbone size w/o insert (bp) 5211
  • Total vector size (bp) 6930
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RT-1306
  • Species
    Synthetic
  • Insert Size (bp)
    1719
  • Promoter T7
  • Tag / Fusion Protein
    • His-tag (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TAATACGACTCACTATAGGG
  • 3′ sequencing primer GCTAGTTATTGCTCAGCGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET30-RT1306 was a gift from Bryan Dickinson (Addgene plasmid # 131521 ; http://n2t.net/addgene:131521 ; RRID:Addgene_131521)

  • For your References section:

    Evolution of a reverse transcriptase to map N(1)-methyladenosine in human messenger RNA. Zhou H, Rauch S, Dai Q, Cui X, Zhang Z, Nachtergaele S, Sepich C, He C, Dickinson BC. Nat Methods. 2019 Dec;16(12):1281-1288. doi: 10.1038/s41592-019-0550-4. Epub 2019 Sep 23. 10.1038/s41592-019-0550-4 PubMed 31548705
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