pFUGW ORANGE Gria1-GFP KI _ mCherry-KASH
(Plasmid #131507)

• Purpose: Endogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)
• Depositing Lab: Harold MacGillavry
• Publication: Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. doi: 10.1371/journal.pbio.3000665.

Backbone

• Vector backbone: pFUGW
• Backbone size w/o insert (bp): 11510
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: gRNA and GFP donor
• gRNA/shRNA sequence: GGGAGCCACAGGATTGTAAC
• Species: M. musculus (mouse), R. norvegicus (rat)
• Entrez Gene: Gria1 (a.k.a. GluA1, gluR-A)
• Entrez Gene: Gria1 (a.k.a. 2900051M01Rik, Glr-1, Glr1, GluA1, GluR-A, GluRA, Glur-1, Glur1, HIPA1, gluR-K1)
• Promoter: hUBC

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)
• 5′ sequencing primer: GGCGAGTGTGTTTTGTGAAG
• 3′ sequencing primer: GCAGCGTATCCACATAGCG

Resource Information

• Supplemental Documents:
  • pFUGW ORANGE Gria1-GFP KI _ mCherry-KASH.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry