pORANGE GFP-CACNA1E KI
(Plasmid #131481)

• Purpose: Endogenous tagging of CaV2.3, R: N-terminal (amino acid position: G5)
• Depositing Lab: Harold MacGillavry
• Publication: Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. doi: 10.1371/journal.pbio.3000665.

Backbone

• Vector backbone: pORANGE
• Backbone size w/o insert (bp): 9252
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: gRNA and GFP donor
• gRNA/shRNA sequence: GCAGGATGGCTCGCTTCGGGG
• Species: M. musculus (mouse), R. norvegicus (rat)
• Entrez Gene: Cacna1e (a.k.a. CACHA1E, Cav2.3)
• Entrez Gene: Cacna1e (a.k.a. A430040I15, BII, Cach6, Cacnl1a6, Cav2.3, Cchra1, alpha1E)
• Promoter: U6 and Cbh

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)
• 5′ sequencing primer: aaggctgttagagagataattggaa
• 3′ sequencing primer: cgggccatttaccgtaagtt

Resource Information

• Supplemental Documents:
  • pORANGE GFP-CACNA1E KI.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry