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Addgene

pCAG-T3-HypaCAS9-pA
(Plasmid #131467)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131467 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGGS
  • Total vector size (bp) 9293
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Codon optimized HypaCas9
  • Species
    Synthetic
  • Insert Size (bp)
    4218
  • Tags / Fusion Proteins
    • Flag (N terminal on insert)
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)
    • polyA (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid vector was constructed by mutation PCR of pCAG-T3-hCAS9-pA plasmid vector (Addgene plasmid 48625) according to the amino acid sequences of HypaCas9 shown in previous study (Chen JS., Enhanced proofreading governs CRISPR-Cas9 targeting accuracy. Nature, 2017).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-T3-HypaCAS9-pA was a gift from Wataru Fujii (Addgene plasmid # 131467 ; http://n2t.net/addgene:131467 ; RRID:Addgene_131467)

  • For your References section:

    High-fidelity endonuclease variant HypaCas9 facilitates accurate allele-specific gene modification in mouse zygotes. Ikeda A, Fujii W, Sugiura K, Naito K. Commun Biol. 2019 Oct 10;2:371. doi: 10.1038/s42003-019-0627-8. eCollection 2019. 10.1038/s42003-019-0627-8 PubMed 31633062
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