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pKLD-CAG-DYSF-CFL
(Plasmid #131457)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131457 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKLD
  • Total vector size (bp) 16156
  • Vector type
    Mammalian Expression, Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    DYSF and Follistatin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6262
  • Mutation
    R15C (in DYSF) and Q2579H (in CFL)
  • Entrez Gene
    DYSF (a.k.a. FER1L1, LGMD2B, LGMDR2, MMD1)
  • Entrez Gene
    FST (a.k.a. FS)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS identified two point mutations: R15C (in DYSF) and Q2579H (in CFL)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKLD-CAG-DYSF-CFL was a gift from Michele Calos (Addgene plasmid # 131457 ; http://n2t.net/addgene:131457 ; RRID:Addgene_131457)

  • For your References section:

    Plasmid-Mediated Gene Therapy in Mouse Models of Limb Girdle Muscular Dystrophy. Guha TK, Pichavant C, Calos MP. Mol Ther Methods Clin Dev. 2019 Oct 14;15:294-304. doi: 10.1016/j.omtm.2019.10.002. eCollection 2019 Dec 13. 10.1016/j.omtm.2019.10.002 PubMed 31890729
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