pGEM-PH-citrine
(Plasmid #131406)

• Purpose: Purpose: synthesis of mRNA encoding a membrane marker. Insert: PH domain of the human phospholipase C delta 1 (PLCD1) fused with the yellow fluorescent protein mCitrine
• Depositing Lab: Lars Hufnagel
• Publication: Guignard et al Science. 2020 Jul 10;369(6500). pii: 369/6500/eaar5663. doi: 10.1126/science.aar5663.

Backbone

• Vector backbone: pGEMHE
• Total vector size (bp): 4356
• Vector type: in vitro transcription

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PH
• Alt name: PLCD1 (PH domain of the human phospholipase C delta 1)
• Species: H. sapiens (human)
• Promoter: T7
• Tag / Fusion Protein:
  • citrine yellow fluorescent protein (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: MluI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • pGEM-PH-citrine.gb
• A portion of this plasmid was derived from a plasmid made by: Dr. Alex McDougall; pRN3-PH-GFP described in doi:10.1242/jcs.00846.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry