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Addgene

pET26b_Nb.b201
(Plasmid #131404)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131404 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET26b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 5656
  • Modifications to backbone
    n/a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Nanobody b201
  • Alt name
    Nb.b201
  • Species
    Synthetic
  • Insert Size (bp)
    1089
  • Promoter T7
  • Tags / Fusion Proteins
    • pelB leader (N terminal on backbone)
    • 6xHis-tag (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET26b_Nb.b201 was a gift from Andrew Kruse (Addgene plasmid # 131404 ; http://n2t.net/addgene:131404 ; RRID:Addgene_131404)

  • For your References section:

    Yeast surface display platform for rapid discovery of conformationally selective nanobodies. McMahon C, Baier AS, Pascolutti R, Wegrecki M, Zheng S, Ong JX, Erlandson SC, Hilger D, Rasmussen SGF, Ring AM, Manglik A, Kruse AC. Nat Struct Mol Biol. 2018 Mar;25(3):289-296. doi: 10.1038/s41594-018-0028-6. Epub 2018 Feb 12. 10.1038/s41594-018-0028-6 PubMed 29434346
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